Extracción de compuestos fenólicos y contenido de catequina en cortezas de tres especies forestales del Cauca-Colombia
Resumen
Se evaluó el contenido de catequina en cortezas de las especies forestales Pinus patula, Pinus oocarpa y Eucaliptus grandis, cultivadas en el Departamento del Cauca-Colombia. Los extractos etanólicos se obtuvieron evaluando las mejores condiciones de tamaño de partícula, tiempo de agitación, relación disolvente-corteza, temperatura y agitación. El contenido de catequina se determinó usando cromatografía líquida de alta resolución (HPLC) con detector ultravioleta y empleando extracción en fase sólida (SPE) para la limpieza de las muestras. Se encontró un contenido de catequina de 2.00±0.03%, 2.96±0.02% y 0.18±0.03% en cortezas de P. patula, P. oocarpa y E. grandis respectivamente. Este contenido de catequina indica el gran potencial en aplicaciones industriales y medicinales para un recurso renovable como la corteza, abundante en la región y cuya disposición en campo afecta ambientalmente las zonas forestales.
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